Comet assay as a procedure for detecting possible genotoxicity induced by non-ionizing radiation
نویسندگان
چکیده
منابع مشابه
Is the Comet Assay a Sensitive Procedure for Detecting Genotoxicity?
Although the Comet assay, a procedure for quantitating DNA damage in mammalian cells, is considered sensitive, it has never been ascertained that its sensitivity is higher than the sensitivity of other genotoxicity assays in mammalian cells. To determine whether the power of the Comet assay to detect a low level of genotoxic potential is superior to those of other genotoxicity assays in mammali...
متن کاملDNA Damage in Leukocytes from Fanconi Anemia (FA) Patients and Heterozygotes Induced by Mitomycin C and Ionizing Radiation as Assessed by the Comet and Comet-FISH Assay
Background: Lymphocytes of Fanconi anemia (FA) show an increased sensitivity to the alkylating agents such as mitomycin C (MMC), but their responses to gamma-irradiation is controversial. The extent of DNA damage in leukocytes of FA patients following irradiation and MMC treatment was studied at cellular and single chromosome level. Methods: DNA damage induced by gamma-rays and MMC was measure...
متن کاملdna damage in leukocytes from fanconi anemia (fa) patients and heterozygotes induced by mitomycin c and ionizing radiation as assessed by the comet and comet-fish assay
background: lymphocytes of fanconi anemia (fa) show an increased sensitivity to the alkylating agents such as mitomycin c (mmc), but their responses to gamma-irradiation is controversial. the extent of dna damage in leukocytes of fa patients following irradiation and mmc treatment was studied at cellular and single chromosome level. methods: dna damage induced by gamma-rays and mmc was measured...
متن کاملDNA damage in leukocytes from Fanconi anemia (FA) patients and heterozygotes induced by mitomycin C and ionizing radiation as assessed by the comet and comet-FISH assay.
BACKGROUND Lymphocytes of Fanconi anemia (FA) show an increased sensitivity to the alkylating agents such as mitomycin C (MMC), but their responses to gamma-irradiation is controversial. The extent of DNA damage in leukocytes of FA patients following irradiation and MMC treatment was studied at cellular and single chromosome level. METHODS DNA damage induced by gamma-rays and MMC was measured...
متن کاملEvaluation of the Comet Assay for Assessing the Dose-Response Relationship of DNA Damage Induced by Ionizing Radiation
Dose- and time-response curves were combined to assess the potential of the comet assay in radiation biodosimetry. The neutral comet assay was used to detect DNA double-strand breaks in lymphocytes caused by γ-ray irradiation. A clear dose-response relationship with DNA double-strand breaks using the comet assay was found at different times after irradiation (p < 0.001). A time-response relatio...
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ژورنال
عنوان ژورنال: Frontiers in Genetics
سال: 2015
ISSN: 1664-8021
DOI: 10.3389/conf.fgene.2015.01.00014